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Anti-Human IgA (α-chain specific)-Peroxidase antibody produced in goat

品牌
Sigma-Aldrich
货号
A0295
规格纯度
affinity isolated antibody
参考价格
5894.34 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Anti-Human IgA (a-chain specific)-peroxidase antibody is isolated from goat anti-human IgA antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the alpha chain of human IgA.Goat antihuman IgA is conjugated to peroxidase and purified to remove unconjugated material. Specificity of the peroxidase conjugated anti-human IgA is determined by immunoelectrophoresis (IEP) and cross-reactivity of the conjugate is determined by enzyme linked immunoassays (ELISA).

特异性

Anti-Human IgA (a-chain specific)-Peroxidase antibody produced in goat conjugate is specific for human IgA when tested against human IgA, IgG, IgM, Bence Jones Kappa and Lambda myeloma proteins. The antibody conjugate does not react with mouse or rat IgG.

免疫原

Purified human IgA.

应用

Anti-Human IgA (α-chain specific) Peroxidase antibody produced in goat has been used in:

  • Enzyme linked immunosorbent assays (ELISA) at dilution of 1:3,000 and 1: 1,600
  • Tissue anti-transglutaminase antibody (tTG) assay at a dilution of 1:1,000
  • Dot blot: a minimum working dilution of 1:80,000 was determined in a direct chemiluminescence assay using 10ng human IgA/dot. Luminol plus enhancer was used as substrate.
  • Immunohistology using formalin-fixed, paraffin-embedded sections at a dilution of 1:100
  • Transcytosis assay of dimeric IgA (D-IgA)
  • Western Blotting

生化/生理作用

IgA is a secretory glycoprotein antibody that regulates the physiological balance between commensal intestinal bacteria and the defenses of the host immune system. It is the highest generated antibody isotype. IgAs are mainly secreted in the intestinal mucosa and prevent the commensal microorganisms from invading the intestines.

外形

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT as preservative

制备说明

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

储存及稳定性

For continuous use, store at 2-8℃ for a maximum of one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD00162417
NACRESNA.46

产品性质

质量水平200
生物来源goat
偶联物peroxidase conjugate
抗体形式affinity isolated antibody
antibody product typesecondary antibodies
克隆polyclonal
形式buffered aqueous solution
species reactivityhuman
浓度4-11 mg/mL
technique(s)direct ELISA: 1:50,000
dot blot: 1:80,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
western blot: suitable
运输dry ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2

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