产品说明

一般描述

Immunoglobulin G (IgG) is part of the immunoglobulin family and is widely expressed in the serum. It consists of a gamma (γ) heavy chain in the constant (C) region. The monomeric structure of IgG consists of two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity (Fab fragments). The third fragment known as fragment crystallizable (Fc).
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody binds to all rabbit Igs.

免疫原

purified rabbit IgG

应用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)
Protein expression in maize endosperm amyloplast preparations or whole cell lysates were analyzed by alkaline phosphatase-conjugated goat anti-rabbit IgG as the secondary antibody.
通过 ELISA 法分析 PADI4 对抗凝血酶的瓜氨酸化作用，使用碱性磷酸酶标记的羊抗兔 IgG 作为在0.05M碳酸盐/碳酸氢盐缓冲液(Ph 9.6)中以1：5000稀释的二次稀释剂。
Goat Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody has been used for ELISA assays at a dilution of 1:5,000.

外形

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂, 50% glycerol, and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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