产品说明
一般描述
The HIS-Select® Filter Plate is an immobilized metal-ion affinity chromatography (IMAC) product that allows rapid purification of small-scale crude cell extracts containing histidine-tagged proteins. The HIS-Select® Filter Plate contains 20 μm, spherical silica particles (100 nm pore size) with a hydrophilic layer.
The HIS-Select® Filter Plate is selective for recombinant proteins with histidine tags and exhibits very low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography. The binding capacity of the HIS-Select® Filter Plate is typically > 2 mg per well as determined with an approximately 30 kDa histidine-tagged protein.
应用
The HIS-Select® Filter Plate is suitable for purification of recombinant proteins with histidine tags.
特点和优势
- High Selectivity for his-tagged proteins
- Ready-to-use plate format
- Compatible with CelLytic™ cell lysis reagents
法律信息
Covered by US Patent No 6,623,655 and are sold for research use only. Commercial use requires additional licenses.
CelLytic is a trademark of sigma-aldrich Co. LLC
HIS-Select is a registered trademark of sigma-aldrich Co. LLC
基本信息
| NACRES | NB.24 |
产品性质
| 等级 | for molecular biology |
| 形式 | plate |
| 储存温度 | 2-8℃ |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H317 - H334 - H341 - H350i - H360D - H372 - H373 - H412 |
| 预防措施声明 | P202 - P260 - P273 - P280 - P302 + P352 - P308 + P313 |
| 危险分类 | Aquatic Chronic 3 - Carc. 1A Inhalation - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 - STOT RE 2 Inhalation |
| 靶器官 | Respiratory Tract |
| 储存分类代码 | 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
