产品说明
一般描述
EZ view Red Protein G Affinity Gel is Protein G covalently bonded to 4% Agarose beads. The affinity gel is used to bind the FC portion of the IgG of an antibody to samples, such as cell lysates and tissue, for purification of proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results.
包装
1 mL in poly bottle
应用
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
特点和优势
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
外形
50% slurry in PBS with 50%Glycerol and 0.0015%
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
法律信息
EZview is a trademark of sigma-aldrich Co. LLC
基本信息
| MDL编号 | MFCD03456743 |
| NACRES | NA.56 |
产品性质
| 形式 | liquid |
| 质量水平 | 200 |
| analyte chemical class(es) | proteins (Immunoglobulins of various mammalian species) |
| technique(s) | affinity chromatography: suitable immunoprecipitation (IP): suitable |
| 容量 | ≥8 mg/mL, gel binding capacity (rabbit IgG) |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
