Anti-Gα_q11, C-Terminal antibody produced in rabbit

产品说明

一般描述

G-proteins are membrane associated heterotrimeric proteins comprising of α-, β-, and γ-subunits. The α-subunit contains a guanine-binding domain that is in an inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the α-subunit from the βγ-subunit complex. This enables the Gα-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the α-subunit with the βγ-subunit complex.

特异性

The sequence is highly conserved among species. The antibody is specific for Gα_q11 and Gα_q.

免疫原

synthetic peptide corresponding to amino acids 350-359 of Gα_q11 or amino acids 344-353 of Gα_q.

应用

Anti-Gα_q11, C-terminal antibody produced in rabbit is suitable for immunoprecipitation and western blot analysis at a working dilution of 1:500-1:2000 using 20μg of rat brain microsomal preparation.

生化/生理作用

G_q α (G₁₁α) regulates the action of phospholipase C (PLC), and results in the release of other intracellular messengers through the inositol phosphate pathway, leading to the release of Ca²⁺ and the activation of phosphorylation.

外形

Solution in phosphate buffered saline, pH 7.4.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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