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Anti-Gα i3 antibody produced in rabbit

品牌
Sigma-Aldrich
货号
G4040
规格纯度
whole antiserum, buffered aqueous solution
参考价格
4692 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

G-proteins are membrane associated heterotrimeric proteins that are comprised of α-, β-, and λ-subunits. These proteins play a key role in signal transduction pathways that regulate cell′s response to several hormones, neuromodulators and ligands.

特异性

Recognizes Gαi3 and Gα0 G-proteins.

免疫原

synthetic peptide corresponding to the C-terminal region of Gαi3.

应用

Anti-Gα i3 antibody produced in rabbit is suitable for immunoprecipitation at a working antibody amount of 4μL using solubilized mouse brain membrane lysates and immunoblotting at a working dilution of 1:2000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates.

生化/生理作用

Gi α 3 is a G-protein subunit that is involved in many signal transduction pathways including the mediation of EGF-induced PLC-γ activation and Ca+2 mobilization in hepatocytes. The α-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the α-subunit from the βλ-subunit complex. This enables the Gα-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the α-subunit with the βλ-subunit complex.

外形

Solution of whole antiserum diluted in PBS, pH 7.4.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD00239679
NACRESNA.41

产品性质

生物来源rabbit
质量水平200
偶联物unconjugated
抗体形式whole antiserum
antibody product typeprimary antibodies
克隆polyclonal
形式buffered aqueous solution
分子量antigen ~40 kDa
species reactivitymouse, hamster, rat, human
technique(s)immunoprecipitation (IP): suitable
western blot: 1:1,000-1:2,000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates
UniProt登记号P08754
运输wet ice
储存温度−20℃
Gene Informationhuman ... GNAI3(2773)
mouse ... Gnai3(14679)
rat ... Gnai3(25643)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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