产品说明
一般描述
RealSeq® is a novel method for preparing small-RNA sequencing libraries that reduces incorporation bias in Next-Generation-Sequencing (NGS). Most bias stems from sequence-dependent variability in the enzymatic ligation reactions that attach the two adapters to the 3′ and 5′ ends of the miRNAs/small RNAs during preparation of sequencing libraries. By using a novel single adapter and circularization, RealSeq® greatly reduces library preparation bias. This technology solves the problem of commonly used sequencing library preparations that lead to underdetection of many miRNAs.
RealSeq®-AC is a small RNA library preparation kit compatible with Illumina Next Generation Sequencing (NGS) technology. RealSeq®-AC is optimized for RNA input between 1ng and 1000ng of total RNA from tissue or cells.
应用
This kit is for small RNA library preparation for NGS. RNA-Seq for small RNA and miRNA; NGS library generation for small RNA and miRNA; RealSeq®-AC is highly efficient, detecting more miRNAs in total RNA samples.
Application Note
RealSeq® accurately quantifies small RNA sequenced on the Illumina Platform. RealSeq®-AC uses ligation of a single adapter producing small RNA libraries with more miRNAs compared to library prep kits using two adapters.
特点和优势
- Significantly reduces sequencing bias in small-RNA sequencing
- Accurately quantifies biologically relevant small RNAs
- Allows for more efficient discovery of novel small RNAs
组分
This kit is for 12 libraries. This product is comprised of the following:
RNA buffer
RealSeq Adapter
RNAse inhibitor
Ligation buffer
Ligase
Blocking Agent
Blocking Enzyme
Blocking Buffer
RealSeq Enzyme
RealSeq Buffer
Dimer Removal Agent
RT Primer
dNTPs
RT buffer
RT Enzyme
PCR Buffer
PCR Polymerase
Rnase Free Water
RealSeq Beads
Reverse Index Primers*
Forward Primer
miRNA Control
Adapter Dilution Buffer
* Index Primers 1-12 are included in REALSEQAC-1KT (500-00012)
See experimental protocol for more information.
储存及稳定性
The three components should be stored at the specified Temperature upon arrival of the Kit.
- RealSeq®-AC (BOX 1) at −20 ℃
- RealSeq®-AC (BOX 2) at −20 ℃
- RealSeq®-AC (Tube with Beads 3) at 2-8 ℃
法律信息
RealSeq is a registered trademark of SomaGenics, Inc.
产品性质
| 运输 | wet ice |
安全信息
| 象形图 |  |
| 危险声明 | H411 |
| 预防措施声明 | P273 |
| 危险分类 | Aquatic Chronic 2 |
| 储存分类代码 | 10 - Combustible liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
