产品说明

一般描述

Lipoprotein, low density (LDL) is synthesized by lipolysis and lipid transfer steps. They are formed from very low-density lipoprotein (VLDL) after uptake by LDL receptor in hepatocytes. LDL normal level of 130 mg/dL is desirable in human plasma. It is referred as bad cholesterol.

应用

Lipoprotein was used to study effect of the surface density of nano-segments immobilized on culture dishes on ex vivo expansion of hematopoietic stem and progenitor cells from umbilical cord blood. It was also used in the separation and cultivation of hematopoietic stem cells from umbilical cord blood by permeation through membranes with nano-segments.
Lipoprotein, low density from human plasma has been used:

• in evaluating its anti-Langerhans cells (LC) differentiation activity in monocyte-derived Langerhans cells (MDLCs)
• in the preparation of oxidized low-density lipoprotein (ox-LDL)
• as a lipoprotein standard in quantifying embryonic cerebrospinal fluid (eCSF) from HH23 embryos by lipid staining

包装

1 vial in glass bottle

生化/生理作用

LDL和HDL负责在血浆中运输饮食和内源胆固醇。LDL是胆固醇和胆固醇脂的主要载体，组成了血浆中总脂蛋白的一半以上。LD通过受体介导内吞作用，被肝脏和其他组织吸收。LDL受体的胞浆结构域帮助形成被小窝，膜上的受体富集区域。受体配体结合域识别LDL上的 apo-B100，导致包被网格蛋白的囊泡形成。ATP依赖质子泵减低囊泡内的pH，导致LDL从它的受体上脱离。当网格蛋白囊泡和溶菌酶融合丧失，会导致多肽和胆固醇脂酶水解。LDL受体会循环至细胞膜。胰岛素，三碘甲状腺原氨酸和地塞米松被证明参与调解LDL受体介导的摄入。
The apolipoprotein component of the LDL particle interacts with extracellular matrix in arterial intima resulting in their retention. LDL is the major driver of atherosclerotic cardiovascular disease progression.

物理属性

低密度脂蛋白比VLDL和IDL(26nm)(MW大约3.5百万)分子质量小，而且更稠密 (~1.04)。LDL蛋白质组分是载脂蛋白 B100。LDL含有20-22%蛋白质，10-15%甘油三酯，20-28%磷酸酯，37-48%胆固醇脂和8-10%胆固醇。

制备说明

Lyophilized from a solution of 0.15 M NaCl and 0.01% EDTA, pH 7.4

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