产品介绍：
产品说明
一般描述
HEPES has been described as one of the best all-purpose buffers available for biological research.[7] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[1]
应用
HEPES has been used:
- As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo†
- As a component of Hanks′ solution used in the degradation testing of Iron†
- As a component of HEPES medium in the transfection of cell culture with plasmids†
- Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells†
- As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies†
基本信息
| 经验(实验)分子式 | C8H18N2O4S |
| 分子量 | 238.30 |
| Beilstein | 883043 |
| MDL编号 | MFCD00006158 |
产品性质
| 质量水平 | 200 |
| 生物来源 | synthetic |
| 等级 | reagent grade |
| 测定 | ≥99% (titration) |
| 形式 | crystalline |
| 有效pH范围 | 6.8 - 8.2 |
| pKa (25 ℃) | 7.5 |
| SMILES string | OCCN1CCN(CC1)CCS(O)(=O)=O |
| InChI | 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14) |
| InChI key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
