产品说明
一般描述
Atto 520 is a novel fluorescent label related to the well-known dye Rhodamine 6G. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. The dye is moderately hydrophilic. Above pH 7.0 the dye exists in an equilibrium with a colourless form (pseudobase). This is a reversible reaction, which does not interfere with the process of coupling to proteins etc. On lowering the pH absorption and fluorescence are restored fully. For details of coupling see our recommended labeling procedures The fluorescence is excited most efficiently in the range 510 - 535 nm. A suitable source of excitation is an Argon-Ion laser using the 514 nm line.
The azide modification is suitable for reactions with alkyne groups (Huisgen reaction - “Click Chemistry“).
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法律信息
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
产品性质
| 测定 | ≥80.0% (HPCE) |
| 质量水平 | 100 |
| 分子量 | Mw 681 g/mol |
| 荧光 | λex 516 nm; λem 545 nm±10 nm in PBS, pH 7.4 |
| 适用性 | suitable for fluorescence |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
