Anti-Corticotropin Releasing Factor antibody produced in rabbit

产品说明

一般描述

Corticotropin Releasing Factor (CRF), a 41 amino acid neuropeptide, belongs to a family of structurally related peptides including urocortin, sauvagine and urotensin I. CRF is derived from a larger 196 amino acids precursor protein (preproCRF). CRF is widely distributed in the brain and peripheral nervous system, with the highest levels in the hypothalamic paraventricular nucleus (PVN).

特异性

The antibody does not cross-react with human or rat CRF (6-33), sheep CRF, rat urocortin, PACAP38, human ACTH, human ACTH (18-39) and sauvagine.

免疫原

synthetic peptide corresponding to the C-terminal of human CRF (amino acids 25-41 with N-terminally added lysine) conjugated to KLH.

应用

Anti-Corticotropin Releasing Factor antibody produced in rabbit has been used in immunohistochemistry (1:2000) and radioimmunoassay.

生化/生理作用

Corticotropin Releasing Factor (CRF) is critical in the regulation of the hypothalamic-pituitary-adrenal gland axis, and autonomic and behavioral responses to stress. CRF effects include changes in motor and cognitive behavior, appetite control and modulation of the immune system. CRF is a potent stimulator of adrenocorticotropic hormone (ACTH) secretion by corticotroph cells from the anterior pituitary in vitro and in vivo. The effects of CRF are mediated through CRF receptor subtypes CRFR1 and CRFR2 (splice variants). CRF binds with a high affinity to CRF-binding protein (CRF-BP), which inactivates CRF, while ligands that dissociate CRF from CRF-BP exhibit cognition-enhancing properties. CRF overproduction has been implicated in anorexia and depression and may lead to Cushing′s syndrome. CRF levels are correlated with Alzheimer′s Disease (AD) pathogenesis.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

储存及稳定性

For continuous use, store at 2-8℃ for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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