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Notch Transcription Factor Inhibitor, SAHM1-Calbiochem

品牌
Sigma-Aldrich
货号
491002
规格纯度
Notch Transcription Factor Inhibitor, SAHM1, is a cell-permeable inhibitor that prevents assembly in the NOTCH1 trans-activation complex.
参考价格
5063.02 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

A cell-permeable, stabilized hydrocarbon-stapled α-helical peptide that targets a critical protein-protein interface and prevents assembly in the NOTCH1 trans-activation complex. Soluble FITC-SAHM1 was found to bind the pre-formed RAMANK-CSL complex (Kd = 0.12 µM) competitively with MAML1, whereas the unmodified FITC-MAML1 (21-36) peptide bound the complex with markedly diminished affinity in an in vitro pull down assay. Treatment of KOPT-K1 cell lines with 20 µM of compound results in the suppression of NOTCH1 target genes, HES4, DTX1, and HES1. A consistent repressive effect on NOTCH1-activated gene expression by this compound was observed across a panel of human T-ALL cell lines, containing diverse mutant NOTCH1 alleles. In HeLa cells, this peptide also inhibited the expression of ICN1 (IC50 = 6.56 µM) dose-dependently in a luciferase reporter-gene assay, as well as that of β-lactamase (IC50<2.5 µM) in a second reporter-gene assay. Furthermore, treatment with this peptide inhibits NOTCH-mediated proliferation in cultured cells at 15 µM, and in a mouse model of T-ALL cells at 30 mg/kg twice daily.

包装

1 mg in Glass bottle

警告

Toxicity: Standard Handling (A)

序列

Acetyl-βAla-ERLRRRI*LCR*HHST

* = Two S5 molecules (a non-natural alkenyl amino acid) stapled together with a double bond

重悬

Following reconstitution, aliquot and freeze (-20℃). Stock solutions are stable for up to 3 months at -20℃.

其他说明

Moellering, R.E., et al. 2009. Nature462, 182.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式C94H162N36O23S · 7C2HF3O2 · 13H2O
分子量3228.95

产品性质

质量水平100
测定≥93% (HPLC)
形式solid
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
颜色 off-white
溶解性DMSO: 50 mg/mL
运输wet ice
储存温度−20℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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