产品说明
一般描述
Monoclonal Anti-MeCP2 (mouse IgG1 isotype) is derived from the Men-8 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the N-terminus of human MeCP2.
Methyl-CpG binding protein 2 (MeCP2) possesses a methyl-CpG-binding domain (MBD) and a transcriptional repression domain.
免疫原
synthetic peptide corresponding to the N-terminus (amino acids 15-30) of human MeCP2.
应用
Monoclonal Anti-MeCP2 antibody has been used:
- in western blotting
- in immunoblotting
- in flow cytometry
- in immunohistofluorescence
- in immunocytochemistry
生化/生理作用
MeCP2 (methyl-CpG binding protein 2) silences transcription by recruiting the histone deacetylase (HDAC) repressive machinery via recruitment of the Sin 3A corepressor, thus removing acetyl groups from histones and consequently, silencing genes.
Methyl-CpG binding protein 2 (MeCP2) has been shown be crucial for brain development. It part of the methyl-CpG-binding proteins, which are involved in repression of gene expression by binding to methylated DNA. Mutations in the gene encoding MeCP2 have been associated with Rett syndrome (RTT).
外形
Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD02686372 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | Men-8, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~75 kDa |
| species reactivity | human, monkey, rat, mouse |
| 包装 | antibody small pack of 25 μL |
| technique(s) | immunocytochemistry: suitable indirect ELISA: suitable microarray: suitable western blot: 1-2 μg/test using nuclear extract of cultured human acute T cell leukemia Jurkat cells or MCF7 cells |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P51608 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... MECP2(4204) mouse ... Mecp2(17257) rat ... Mecp2(29386) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
