产品说明
一般描述
Bolton Broth Selective Supplement is a mixture of four different antibiotics in lyophilized form. Vancomycin, Cefoperazone, and Trimethoprim inhibit the growth of Gram-positive and Gram-negative bacteria. Amphotericin B largely reduces the growth of Yeasts and Molds.
应用
Bolton Broth Selective Supplement is an additive used during the preparation of Bolton Selective Enrichment Broth for the enrichment of Campylobacter from foods.
联系
Replaces: 1.00079.0017
分析说明
Growth promotion test according to ISO 11133
Mixed inoculum of Campylobacter, E.coli and P.mirabilis.
Additionaly monoculture of E.coli and P.mirabilis.
Afterwards confirmation on mCCDA resp. Tryptic soy agar
Inoculum on reference medium
- Campylobacter coli ATCC 43478 (WDCM 00004):
10 - 100
- Campylobacter jejuni ATCC 33291 (WDCM 00005): 10 - 100
- Campylobacter jejuni ATCC 29428 (WDCM 00156): 10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013): ≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012): ≥ 1000
- Proteus mirabilis ATCC 29906 (WDCM 00023): ≥ 1000
Growth on mCCDA
- Campylobacter coli ATCC 43478 (WDCM 00004):
>10 CFU
- Campylobacter jejuni ATCC 33291 (WDCM 00005): >10 CFU
- Campylobacter jejuni ATCC 29428 (WDCM 00156): >10 CFU
Growth on Tryptic soy agar
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Proteus mirabilis ATCC 29906 (WDCM 00023): no growth
Colony colour
- Campylobacter coli ATCC 43478 (WDCM 00004):
greyish
- Campylobacter jejuni ATCC 33291 (WDCM 00005): greyish
- Campylobacter jejuni ATCC 29428 (WDCM 00156): greyish
Incubation:
Bolton broth: 5 hours at 44°C, afterwards 44 hours at 41.5°C, Anaerocult C
Confirmation on mCCDA: at least 44 hours at 41.5°C, Anaerocult C
Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic
产品性质
| 质量水平 | 200 |
| agency | according to ISO 10272-1 |
| reg. compliance | FDA -BAM |
| 形式 | lyophilized powder |
| 组成 | Amphotericin B, 5 mg Cefoperazone, 10 mg Trimethoprim, 10 mg Vancomycin, 10 mg |
| 包装 | pkg of 10 vials |
| technique(s) | microbiological culture: suitable |
| application(s) | food and beverages microbiology pathogen testing |
| 储存温度 | 2-8℃ |
| 适用性 | Campylobacter spp. Listeria spp. |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H317 - H334 - H361d - H373 |
| 预防措施声明 | P201 - P202 - P280 - P302 + P352 - P304 + P340 + P312 - P308 + P313 |
| 危险分类 | Repr. 2 - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 2 |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
