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ELASTIN staining solution acc. to Weigert

品牌
Sigma-Aldrich
货号
1.00591
包装型号
规格纯度
for microscopy
参考价格
1420.72 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

The ELASTIN staining solution acc. to Weigert - for microscopy, is a ready-to-use solution used to detect elastic fibers in connective tissues. Elastic connective-tissue fibers are bundles of glycoprotein-containing microfibrils that accumulate the polymeric protein elastin in their development, gradually resulting in the formation of coarse elastic fibers and membranes. The microfibrils on the surface of the elastic connective-tissue fibers expose high numbers of disulfide groups, which can be chemically detected when sufficient material is available for light-microscopic investigations. Furthermore, they are strongly acidic and thus bind the resorcin-fuchsin dye by electropolarity via interface adsorption.
The resorcin-fuchsin staining method is a regressive method. In tissue sections, nuclei can be stained using a 0.1% nuclear fast red-aluminum sulfate solution (Product number 1.00121) if not only elastic fibers, but also nuclei and the background also need to be visualized.
Brilliant staining results of these fibres are usually achieved when using the ELASTIN staining solution acc. to Weigert - for microscopy in combination with the Picrofuchsin solution acc. van Gieson - for microscopy, Product number 1.00199 and nuclear staining with Weigert′s iron hematoxylin kit - for nuclear staining in histology, Product number 1.15973 respectively Nuclear fast red-aluminium sulfate solution 0.1% - for microscopy, Product number 1.00121.
The 500ml bottle is sufficient for 400 - 500 applications. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

分析说明

Suitability for microscopy: passes test
Nuclei: blue-brown
Collagen: red
Muscle fibers: yellow
Cytoplasm: yellow
Elastic fibers: black

产品性质

蒸汽压59 hPa ( 20 ℃)
质量水平400
形式liquid
expl. lim.3.5-15 % (v/v) ethanol)
IVDfor in vitro diagnostic use
bp78.3 ℃/1013 hPa (ethanol)
转变温度flash point 18 ℃
密度0.84 g/cm3 at 20 ℃
application(s)clinical testing
diagnostic assay manufacturing
hematology
histology
储存温度2-8℃

安全信息

象形图GHS02GHS05
警示用语:Danger
危险声明H225 - H290 - H319
预防措施声明P210 - P233 - P234 - P240 - P241 - P305 + P351 + P338
危险分类Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1
储存分类代码3 - Flammable liquids
WGKWGK 3
闪点(F)64.4 °F
闪点(C)18 ℃

Sigma-Aldrich

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