产品说明
一般描述
Nestin is a class VI intermediate filament (IF) protein. It has been reported that nestin expression is significantly increased in melanoma and correlated with more advanced stages of the disease. An immunohistochemical analysis identified nestin-positive cells in 84% (35/42) of primary melanoma and 83% (10/12) of metastatic melanoma. Nestin immunoreactivity was also observed in melanoma cells in all (10/10) cases of HMB-45-negative amelanotic and melanotic, non-desmoplastic melanoma. Nestin expression has been reported in tumors of the CNS, including astrocytoma, ependymoma, oligodendroglioma, glioblastoma, and primitive neuroectodermal tumors. Nestin has been detected in human gliomas, including low and high grade, but its expression is observed more frequently in high grade than in low grade gliomas. Nestin overexpression is seen in carcinomas, including prostatic adenocarcinoma, pancreatic ductal carcinoma, thyroid carcinoma, and in mesenchymal tumors such as GIST and DFSP. Among the breast carcinoma subtypes, nestin is highly expressed in basal breast cancer subtype (ERα-/PR-/Her2-) but not in the Her2 subtype (ERα-/PR-/Her2+) or luminal epithelial phenotype (ERα+/PR+). Only cytoplasmic staining is considered positive, whereas any nuclear staining is considered as background artifact. In normal skin, nestin is observed in endothelial cells and the bulge area of hair follicles.
质量
 IVD |  IVD |  IVD |  RUO |
联系
Nestin Positive Control Slides, Product No. 388S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).
外形
Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
制备说明
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其他说明
For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com
法律信息
Cell Marque is a trademark of sigma-aldrich Co. LLC
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 500 100 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | culture supernatant |
| antibody product type | primary antibodies |
| 克隆 | 10C2, monoclonal |
| 描述 | For In Vitro Diagnostic Use in Select Regions (See Chart) |
| 形式 | buffered aqueous solution |
| species reactivity | human |
| 包装 | vial of 0.1 mL concentrate (388M-14) vial of 0.5 mL concentrate (388M-15) bottle of 1.0 mL predilute (388M-17) vial of 1.0 mL concentrate (388M-16) bottle of 7.0 mL predilute (388M-18) |
| manufacturer/tradename | Cell Marque™ |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100 |
| 同位素/亚型 | IgG1 |
| 控制 | tonsil |
| 运输 | wet ice |
| 储存温度 | 2-8℃ |
| 可视化 | cytoplasmic |
