应用
1,3-Bis[tris(hydroxymethyl)methylamino]propane is used as a buffer for polymerase chain reaction and molecular biology. It is also used with hydrochloric acid buffer for stabilization of farnesyl diphosphate isolated from a strain of saccharomyces cerevisiae. It acts as a ligand and forms dinuclear hydroxo complexes with Lanthanides(III). Further, it is useful for calibration of genetically encoded pH indicators expressed in the cytosol or mitochondria. It is also employed to investigate the influence of anions with varying nucleophilic properties on the autoxidation of oxymyoglobin. In addition to this, it is used in the purification of glucose-binding protein from membranes of sulfolobus solfataricus and as a non-zwitterionic buffer, which is used in the pH range between 6.3 and 9.5.

备注
pKa{1}=6.8, useful pH range 6.3-9.5Incompatible with strong oxidizing agents.

基本信息

MDL
MFCD00004689

EINECS
264-899-3

分子式
CH₂[CH₂NHC(CH₂OH)₃]₂

分子量
282.34

熔点
168-170°

灵敏度
Ambient temperatures.

形态
Powder

溶解性
Soluble in water.

安全信息

TSCA
否