Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A(MGAT4A)ELISA检测试剂盒采用双抗体夹心酶联免疫吸附检测方法。向预包被了抗体的微孔板中，加入标准品和待测样本，温育后，加入生物素标记抗体和过氧化物酶标记的链霉亲和素。经过温育和洗涤结合形成的免疫复合物，去除未结合的酶，然后加入显色底物TMB。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的待测因子浓度呈正相关。最后，在450 nm处测定反应孔样品吸光度（OD）值，通过绘制标准曲线计算出样本待测因子的浓度。

血清、血浆、细胞培养上清液和组织等。

双抗夹心法。

MGAT4A；GNT-IV；GNT-IVA；GnT-4a；alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A；N-acetylglucosaminyltransferase IVa；N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa；UDP-GlcNAc:a-1,3-D-mannoside b-1,4-acetylglucosaminyltransferase IV；UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa；UDP-N-acetylglucosamine:alpha1,3-d-mannoside beta1,4-N-acetylglucosaminyltransferase；alpha-1,3-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase；glcNAc-T IVa；mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isoenzyme A；mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A