B细胞活化因子(BAFF;CD257)ELISA检测试剂盒采用双抗体夹心酶联免疫吸附检测方法。向预包被了抗体的微孔板中，加入标准品和待测样本，温育后，加入生物素标记抗体和过氧化物酶标记的链霉亲和素。经过温育和洗涤结合形成的免疫复合物，去除未结合的酶，然后加入显色底物TMB。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的待测因子浓度呈正相关。最后，在450 nm处测定反应孔样品吸光度（OD）值，通过绘制标准曲线计算出样本待测因子的浓度。

血清、血浆、细胞培养上清液和组织等。

双抗夹心法。

TNFSF13B；BAFF；BLYS；CD257；DTL；TALL-1；TALL1；THANK；TNFSF20；TNLG7A；ZTNF4；TNF superfamily member 13b；tumor necrosis factor ligand superfamily member 13B；ApoL related ligand TALL-1；B-cell-activating factor；B-lymphocyte stimulator；Delta4 BAFF；TNF and ApoL-related leukocyte expressed ligand 1；TNF homolog that activates apoptosis；delta BAFF；dendritic cell-derived TNF-like molecule；epididymis secretory sperm binding protein；tumor necrosis factor (ligand) superfamily, member 13b；tumor necrosis factor (ligand) superfamily, member 20；tumor necrosis factor ligand 7A；tumor necrosis factor superfamily member 13b；tumor necrosis factor-like protein ZTNF4；B细胞活化因子(BAFF;CD257)；B细胞活化因子(BAFF)；B淋巴细胞刺激因子(BLyS)；B-细胞激活因子(BAFF)；B细胞活化因子(BAFF/TNFSF13B)；B细胞活化因子；重组B细胞活化因子(BAFF)；B细胞刺激因子；B-细胞启动因子