TNFRSF25/DR3 ELISA检测试剂盒采用双抗体夹心酶联免疫吸附检测方法。向预包被了抗体的微孔板中，加入标准品和待测样本，温育后，加入生物素标记抗体和过氧化物酶标记的链霉亲和素。经过温育和洗涤结合形成的免疫复合物，去除未结合的酶，然后加入显色底物TMB。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的待测因子浓度呈正相关。最后，在450 nm处测定反应孔样品吸光度（OD）值，通过绘制标准曲线计算出样本待测因子的浓度。

血清、血浆、细胞培养上清液和组织等。

双抗夹心法。

TNFRSF25；APO-3；DDR3；DR3；GEF720；LARD；PLEKHG5；TNFRSF12；TR3；TRAMP；WSL-1；WSL-LR；TNF receptor superfamily member 25；tumor necrosis factor receptor superfamily member 25；Guanine nucleotide exchange factor 720；PH domain-containing family G member 5；Pleckstrin homology domain-containing family G member 5；apoptosis inducing receptor；apoptosis-inducing receptor AIR；apoptosis-mediating receptor DR3；apoptosis-mediating receptor TRAMP；death domain receptor 3 soluble form；death receptor beta；lymphocyte-associated receptor of death；protein WSL-1；tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein)