M-07e(人巨细胞白血病细胞)(STR鉴定正确)
| 货号: | CL-0686 | | 包装: | 1×106cells/T25培养瓶 |
产品介绍
| 种属 | 人类 | | 年龄(性别) | 女性，6个月 | | 组织来源 | 外周血 | | 生长特性 | 悬浮细胞 | | 细胞形态 | 淋巴母细胞样 | | 背景描述 | Established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-beta, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells. Exome and RNA sequence data are available | | 生物安全等级 | 1 | | 生长培养基 | RPMI-1640+10% FBS+8ng/mL GM-CSF+1% P/S | | 推荐传代比例 | 5×105-1×106cells/mL | | 推荐换液频率 | 2～3次/周 | | 倍增时间 | ～40小时 | | 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮 | | 培养条件 | 气相：空气，95%；CO2，5%温度：37℃ | | 抗原表达情况 | CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR - | | 注意事项 | 该细胞为悬浮细胞，请注意离心收集细胞悬液；请勿直接倒掉细胞培养液。 | | 保藏机构 | DSMZ;ACC-104 |
STR鉴定 STR位点信息 | Amelogenin | X | | CSF1PO | 9,10 | | D2S1338 | 19,25 | | D3S1358 | 16,19 | | D5S818 | 11 | | D7S820 | 11 | | D8S1179 | 13,16 | | D13S317 | 10,11 | | D16S539 | 11 | | D18S51 | 15,20 | | D19S433 | 14,15 |
| D21S11 | 30,32.2 | | FGA | 18,22 | | PentaD | 9 | | PentaE | 12,14 | | TH01 | 6,8 | | TPOX | 8 | | vWA | 16,18 | | D6S1043 | 13,19 | | D12S391 | 23,24 | | D2S441 | 14 |
STR鉴定图 
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