VERO非洲绿猴肾细胞

产品介绍

目录号 Serial	SCSP-520
标识符 Identifier	CSTR:19375.09.3101MONSCSP520
描述 Description	Vero细胞株是日本千叶大学Y. Yasumura和Y. Kawakita从正常成年非洲绿猴的肾脏建株的。1964年6月15日，B. Simizu将其从千叶大学带到美国国立卫生研究院(NIH)过敏及传染病研究所热带病毒实验室时，已传至第93代。该细胞是贴壁依赖性的成纤维细胞。它能支持多种病毒的增殖，包括乙型脑炎、脊髓灰质炎、狂犬病等病毒，已被准许用于生产人用病毒疫苗。细胞质量控制： 1.支原体检测结果：每个批次均通过本库支原体检测，结果为阴性。 2.STR鉴定结果： ①该株细胞DNA进行小鼠细胞STR分型结果显示，扩增后图谱清晰，分型结果良好； ②该细胞为猴源Vero细胞，未发现存在人或其他猴源细胞污染。 3.种属鉴定结果：采用PCR引物扩增技术对该细胞株进行种属鉴定，结果显示为非洲绿猴细胞，且无其他常见物种细胞污染。
动物种别 Organism	非洲绿猴
性别 Gender
组织来源 Tissue and Cell Type	肾脏
形态 Morphology	上皮样，贴壁生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	非洲绿猴肾细胞Vero完全培养液配方(100 ml)： MEM (Invitrogen, 11090081) 87 ml FBS (Gibco) 10 ml Glutamax(invitrogen 35050061) 1 ml SODIUM PYRUVATE SOL(invitrogen 11360070) 1 ml Non-essential Amino Acids, 100X (Invitrogen, 11140050) 1 ml 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。生物安全等级：BSL-1 参考传代周期：2～3天参考传代比例：1：3～1：6 参考换液频率：每周2～3次冻存液配方：完全培养液95%，DMSO 5%
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.Yasumura Y, Kawakita Y. Studies on SV40 in tissue culture - preliminary step for cancer research in vitro. Nihon Rinsho 21: 1201-1215, 1963.Simizu B, et al. Characterization of the Tacaribe group of arboviruses. I. Propagation and plaque assay of Tacaribe virus in a line of African green monkey kidney cells (Vero). Proc. Soc. Exp. Biol. Med. 125: 119-123, 1967. PubMed: 6027511Rhim JS, Schell K. Cytopathic and plaque assay of rubella virus in a line of African green monkey kiency cells (Vero). Proc. Soc. Exp. Biol. Med. 125: 602-606, 1967. PubMed: 4961492Liebhaber H, et al. Replication of rubella virus in a continuous line of African green monkey kidney cells (Vero). Proc. Soc. Exp. Biol. Med. 125: 636-643, 1967. PubMed: 4961494Sasaki K, et al. Studies on measles virus. II. Propagation in two established simian renal cell lines and development of a plaque assay. Kitasato Arch. Exp. Med. 37: 27-42, 1964. PubMed:5833688Earley E, et al. A plaque neutralization method for arboviruses. Proc. Soc. Exp. Biol. Med. 125(3): 741-747, 1967. PubMed:15938255Rhim JS, et al. Temperature dependence of the synthesis of adenovirus tumor and viral antigens. Proc. Soc. Exp. Biol. Med. 127: 642-646, 1968. PubMed: 5689485Rhim JS, Schell K. Cytopathic effects of the parainfluenza virus SV5 in Vero cells. Nature 216: 271-272, 1967. PubMed: 4293683Ozawa Y. Studies on the replication of African horse-sickness virus in two different cell line cultures. Arch. Gesamte Virusforsch. 21: 155-169, 1967. PubMed: 4232530Rhim JS, et al. Growth of Junin virus, the etiological agent of Argentinian hemorrhagic fever, in cell cultures. Arch. Gesamte Virusforsch. 21: 243-252, 1967. PubMed: 5591575Huber M, et al. Tyrosine phosphorylation events during coxsackievirus B3 replication. J. Virol. 71: 595-600, 1997. PubMed: 8985388Pugachev KV, et al. Improvement of the specific infectivity of the rubella virus (RUB) infectious clone: determinants of cytopathogenicity induced by RUB map to the nonstructural proteins. J. Virol. 71: 562-568, 1997. PubMed: 8985384Mundt W, et al. Perfusion system and a method for the large scale production of virus or virus antigen. US Patent 5,719,051 dated Feb 17 1998Nichol PF, et al. Herpes simplex virus gene expression in neurons: viral DNA synthesis is a critical regulatory event in the branch point between the lytic and latent pathways. J. Virol. 70: 5476-5486, 1996. PubMed: 8764059Govorkova EA, et al. African green monkey kidney (Vero) cells provide an alternative host cell system for influenza A and B viruses. J. Virol. 70: 5519-5524, 1996. PubMed: 8764064White LJ, et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293Martinez R, et al. Herpes simplex virus type 1 alkaline nuclease is required for efficient processing of viral DNA replication intermediates. J. Virol. 70: 2075-2085, 1996. PubMed: 8642627Zeng L, et al. Identification of amino acids involved in a recognition by dengue virus NS3-specific, HLA-DR15-restricted cytotoxic CD4+ T-cell clones. J. Virol. 70: 3108-3117, 1996. PubMed: 8627790Hill JM, et al. In vivo epinephrine reactivation of ocular herpes simplex virus type 1 in the rabbit is correlated to a 370-base-pair region located between the promoter and the 5' end of the 2.0-kilobase latency-associated transcript. J. Virol. 70: 7270-7274, 1996. PubMed: 8794381Carter KL, et al. Characterization of the products of the UL43 gene of herpes simplex virus 1: potential implications for regulation of gene expression by antisense transcription. J. Virol. 70: 7663-7668, 1996. PubMed: 8892886Malik AK, Weller SK. Use of transdominant mutants of the origin-binding protein (UL9) of herpes simplex virus type 1 to define functional domains. J. Virol. 70: 7859-7866, 1996. PubMed:8892908Chen Y, et al. Demonstration of binding of dengue virus envelope protein to target cells. J. Virol. 70: 8765-8772, 1996. PubMed:8971005Sandri-Goldin RM, Hibbard MK. The herpes simplex virus type 1 regulatory protein ICP27 coimmunoprecipitates with anti-sm antiserum, and the C terminus appears to be required for this interaction. J. Virol. 70: 108-118, 1996. PubMed: 8523514Carter KL, Roizman B. The promoter and transcriptional unit of a novel herpes simplex virus 1 alpha gene are contained in, and encode a protein in frame with, the open reading frame of the alpha22 gene. J. Virol. 70: 172-178, 1996. PubMed: 8523523Russell DW, Miller AD. Foamy virus vectors. J. Virol. 70: 217-222, 1996. PubMed: 8523528Lukonis CJ, Weller SK. Characterization of nuclear structures in cells infected with herpes simplex virus type 1 in the absence of viral DNA replication. J. Virol. 70: 1751-1758, 1996. PubMed:8627697Lagunoff M, et al. Phenotypic properties of herpes simplex virus 1 containing a derepressed open reading frame P gene. J. Virol. 70: 1810-1817, 1996. PubMed: 8627705Uprichard SL, Knipe DM. Herpes simplex ICP27 mutant viruses exhibit reduced expression of specific DNA replication genes. J. Virol. 70: 1969-1980, 1996. PubMed: 8627723Leopardi R, Roizman B. The herpes simplex virus major regulatory protein ICP4 blocks apoptosis induced by the virus or by hyperthermia. Proc. Natl. Acad. Sci. USA 93: 9583-9587, 1996. PubMed: 8790373Pereira ME, et al. Invasive phenotype of Trypanosoma cruzi restricted to a population expressing trans-sialidase. Infect. Immun. 64: 3884-3892, 1996. PubMed: 8751943AOAC International Virus in beef (ground), microbiological method. Gaithersburg, MD: AOAC International; AOAC "Official Methods of Analysis of the AOAC International" 975.56.Nucleofection technology from LonzaSingle-use syringes (sterile) for the injection of 100 units per millilitre insulin (U-100), Appendix O. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 1077-1992.Single-use(sterile) infusion sets for general medical use. Appendix F, Method of test for cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 2385-1990.Medical equipment--Single-use winged intravenous devices (sterile) for general medical use. Appendix J. Method of test for cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 2485:1995.Medical devices--Polymer urethral catherters for general medical use. Appendix B. Method of testing catheters for cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 2696:1996.General requirements for single-use, sterile, plasticized polyvinyl chloride (PVC) packs for human blood. Part2: Multiple blood pack systems. Appendix Q. Method of test for Cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 3787.2-1997.Testing of products for use in contact with drinking water. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 4020:2002.Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS ISO 10993.5-2002.Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10993-5:1999.Test for absence of mycoplasmas. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI (Vet) 3, 2003Standard Practice for Indirect Detection of Mycoplasma in Cell Culture by 4-6-Diamidino-2-2 Phenylindole (DAPI) Staining. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1533-00 (Reapproved 2006).Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Fingerpads of Adult Volunteers. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1838-02.Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02.

广州伟伯科技有限公司
手机: 13533759269
联系人: 林生