CHO-K1中国仓鼠卵巢细胞亚株

产品介绍

目录号 Serial	SCSP-507
标识符 Identifier	CSTR:19375.09.3101HAMSCSP507
描述 Description	1957年，T. T. Puck取成年中国仓鼠的卵巢活体组织建立CHO细胞，而CHO-K1正是CHO细胞株的亚克隆。该细胞易于转染，生长时需要在培养基中加入脯氨酸。每个批次均通过本库支原体检测，结果为阴性。质量控制：支原体检测结果：每个批次均通过本库支原体检测，结果为阴性。种属鉴定结果：采用PCR引物扩增技术对该细胞株进行种属鉴定，结果显示为中国仓鼠细胞，且无其他常见物种细胞污染。
动物种别 Organism	中国仓鼠
性别 Gender	雌性
组织来源 Tissue and Cell Type	卵巢
形态 Morphology	上皮样，贴壁生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	中国仓鼠卵巢细胞亚株CHO-K1完全培养液配方： F-12K (Invitrogen，21127-022) 89 ml FBS (Gibco) 10 ml Glutamax (Invitrogen, 35050061) 1 ml 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。生物安全等级：BSL-1 参考传代周期：2-3天参考传代比例：1:3-1:5 参考换液频率：2-3天冻存液配方：完全培养液95%，DMSO 5%
供应限制 Permits and Restrictions	A。仅供研究之用。
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