PC-12大鼠嗜铬细胞瘤细胞(未分化)

产品介绍

目录号 Serial	SCSP-517
标识符 Identifier	CSTR:19375.09.3101RATSCSP517
描述 Description	该细胞系来自能移植的雄性大鼠肾上腺嗜铬细胞瘤。这些细胞表达神经生长因子(NGF)受体。细胞铺在经四型胶原酶包被的培养瓶上，可逆地响应NGF诱导产生神经表型。这些细胞不合成肾上腺素。每个批次均通过本库支原体检测，结果为阴性。经我库诱导分化检测，在NGF诱导下产生神经表型。
动物种别 Organism	大鼠
性别 Gender
组织来源 Tissue and Cell Type	肾上腺
形态 Morphology	成团状悬浮、半贴壁；小的形状不规则细胞
培养基和添加剂 Complete Growth Medium and Culture Conditions	大鼠嗜铬细胞瘤细胞PC-12 完全培养液(100 ml)： RPMI Medium 1640 (Invitrogen, 11875-093) 85 ml FBS (Gibco) 5 ml heat-inactivated horse serum (GIBCO, 26050-070)10 ml 参考传代周期：3-4天参考传代比例：1:2-1:4 【注意：】按照5×1051×106个/ml的密度传代。PC12细胞呈漂浮成簇状生长，伴有少量松散贴壁的细胞，换液、传代时需小心保留漂浮生长的细胞。参考换液频率：2-3天冻存液配方：完全培养液95%，DMSO 5% 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed:3839317Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125U.S. Pharmacopeia USP Monographs: Small Intestinal Submucosa Wound Matrix. Rockville, MD. USP32-NF27, 2005Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897

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