DU 145人前列腺癌细胞(2017年新引进)

产品介绍

目录号 Serial	SCSP-5024
标识符 Identifier	CSTR:19375.09.3101HUMSCSP5024
描述 Description	人前列腺癌细胞DU 145是从一位69岁白人前列腺癌患者的脑部转移病灶中建株的。该细胞株几乎检测不到激素敏感性，仅仅酸性磷酸酶呈弱阳性，分离得到的细胞在软琼脂中可形成克隆。该细胞不表达前列腺抗体。对该细胞株及原始肿瘤细胞的亚显微结构分析可见微绒毛、微丝及细胞桥粒、线粒体、发育良好的高尔基体和异质溶酶体。每个批次均通过本库支原体检测，结果为阴性。在我库通过STR鉴定无误。STR鉴定结果为：D7S820:7,10,11; D13S317:12,13,14; D5S818:10,13; vWA:17,18,19; THO1:7; CSF1PO:10,11; TPOX:11; D16S539:11,13; Amelogenin:X,Y
动物种别 Organism	人；白人
性别 Gender	男性，69岁
组织来源 Tissue and Cell Type	前列腺；来源于转移灶：大脑
形态 Morphology	上皮样，贴壁生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	人前列腺癌细胞DU145完全培养液配方(100 ml)： MEM (Invitrogen，11090081) 88 ml FBS (Gibco)10 ml Glutamax(invitrogen 35050061) 1 ml Sodium Pyruvate 100 mM Solution(invitrogen 11360070) 1 ml 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	Papsidero LD, et al. Prostate antigen: a marker for human prostate epithelial cells. J. Natl. Cancer Inst. 66: 37-42, 1981. PubMed: 6935463Stone KR, et al. Isolation of a human prostate carcinoma cell line (DU 145). Int. J. Cancer 21: 274-281, 1978. PubMed: 631930Mickey DD, et al. Heterotransplantation of a human prostatic adenocarcinoma cell line in nude mice. Cancer Res. 37: 4049-4058, 1977. PubMed: 908039Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991Sheng S, et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. PubMed:8876194Carter RE, et al. Prostate-specific membrane antigen is a hydrolase with substrate and pharmacologic characteristics of a neuropeptidase. Proc. Natl. Acad. Sci. USA 93: 749-753, 1996. PubMed: 8570628Nupponen NN, et al. Genetic alterations in prostate cancer cell lines detected by comparative genomic hybridization. Cancer Genet. Cytogenet. 101: 53-57, 1998. PubMed: 9460501Robinson D, et al. A tyrosine kinase profile of prostate carcinoma. Proc. Natl. Acad. Sci. USA 93: 5958-5962, 1996. PubMed:8650201Su ZZ, et al. Surface-epitope masking and expression cloning identifies the human prostate carcinoma tumor antigen gene PCTA-1 a member of the galectin gene family. Proc. Natl. Acad. Sci. USA 93: 7252-7257, 1996. PubMed: 8692978Zhu X, et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224Boffa LC, et al. Invasion of the CAG triplet repeats by a complementary peptide nucleic acid inhibits transcription of the androgen receptor and TATA-binding protein genes and correlates with refolding of an active nucleosome containing a unique AR gene sequence. J. Biol. Chem. 271: 13228-13233, 1996. PubMed:8662737DU 145 was isolated by K.R. Stone et al from a lesion in the brain of a patient with metastatic carcinoma of the prostate and a 3 year history of lymphocytic leukemia.

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