293T人胚肾细胞

产品介绍

目录号 Serial	GNHu17
标识符 Identifier	CSTR:19375.09.3101HUMGNHu17
描述 Description	293细胞株插入SV40 T-抗原的温度敏感基因后产生的高转染效率的衍生株称为293T。在本库通过支原体检测。在本库通过STR检测。
动物种别 Organism	人
性别 Gender	***
组织来源 Tissue and Cell Type	肾
形态 Morphology	上皮细胞
培养基和添加剂 Complete Growth Medium and Culture Conditions	DMEM培养基(GIBCO by life technologies ,货号C11995500BT)，90%；优质胎牛血清，10%。气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	Sena-Esteves M , et al. Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons. J. Virol. 73: 10426-10439, 1999. PubMed: 10559361Pensiero M , et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 5,952,225 dated Sep 14 1999Pensiero M , et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 6,329,199 dated Dec 11 2001Pear WS , et al. Production of High-Titer Helper-Free Retroviruses by Transient Transfection. Proc. Natl. Acad. Sci. USA 90: 8392-8396, 1993. PubMed: 7690960

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