热休克蛋白90(Hsp-90)ELISA检测试剂盒采用双抗体夹心酶联免疫吸附检测方法。向预包被了抗体的微孔板中，加入标准品和待测样本，温育后，加入生物素标记抗体和过氧化物酶标记的链霉亲和素。经过温育和洗涤结合形成的免疫复合物，去除未结合的酶，然后加入显色底物TMB。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的待测因子浓度呈正相关。最后，在450 nm处测定反应孔样品吸光度（OD）值，通过绘制标准曲线计算出样本待测因子的浓度。

血清、血浆、细胞培养上清液和组织等。

双抗夹心法。

HSP90AA1；EL52；HEL-S-65p；HSP86；HSP89A；HSP90A；HSP90N；HSPC1；HSPCA；HSPCAL1；HSPCAL4；HSPN；Hsp103；Hsp89；Hsp90；LAP-2；LAP2；heat shock protein 90 alpha family class A member 1；heat shock protein HSP 90-alpha；HSP 86；LPS-associated protein 2；epididymis luminal secretory protein 52；epididymis secretory sperm binding protein Li 65p；heat shock 86 kDa；heat shock 90kD protein 1, alpha；heat shock 90kD protein 1, alpha-like 4；heat shock 90kD protein, alpha-like 4；heat shock 90kDa protein 1, alpha；heat shock protein 90kDa alpha (cytosolic), class A member 1；heat shock protein 90kDa alpha family class A member 1；lipopolysaccharide-associated protein 2；renal carcinoma antigen NY-REN-38；热休克蛋白90(Hsp-90)；热休克蛋白90a(HSP-90a)；热激蛋白-90(HSP-90)；热休克蛋白90(HSP90)；热休克蛋白-90(HSP-90)；热休克蛋白-90α(HSP-90α)；热休克蛋白90-alpha(Hsp90aa1/Hsp86/Hspca)；热休克蛋白90α(HSP-90α)；90kDa热休克蛋白αA1