热休克蛋白47(HSP47)ELISA检测试剂盒采用双抗体夹心酶联免疫吸附检测方法。向预包被了抗体的微孔板中，加入标准品和待测样本，温育后，加入生物素标记抗体和过氧化物酶标记的链霉亲和素。经过温育和洗涤结合形成的免疫复合物，去除未结合的酶，然后加入显色底物TMB。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的待测因子浓度呈正相关。最后，在450 nm处测定反应孔样品吸光度（OD）值，通过绘制标准曲线计算出样本待测因子的浓度。

血清、血浆、细胞培养上清液和组织等。

双抗夹心法。

SERPINH1；AsTP3；CBP1；CBP2；HSP47；OI10；PIG14；PPROM；RA-A47；SERPINH2；gp46；serpin family H member 1；serpin H1；47 kDa heat shock protein；arsenic-transactivated protein 3；cell proliferation-inducing gene 14 protein；collagen binding protein 1；colligin-1；colligin-2；heat shock protein 47；rheumatoid arthritis antigen A-47；rheumatoid arthritis-related antigen RA-A47；serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)；serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2)；serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)；热休克蛋白47(HSP47)